The signal curve in its exponential phase. Real-time PCR data are analyzed after setting an arbitrary threshold that must intersect The MBCF sells disposables for use in qPCR, such as: You must bring your own supplies (tips, tubes, gloves).The MBCF provides the SYBR Green, if needed.Unless the plate is sealed prior to bringing to MBCF, you must use a dUTPase system.We strongly recommend that you sequence the amplicon you obtain to make sure this.You must provide the Genbank Accession number of your gene of interest.
RESERVE: Email us to check availability and reserve time on the Cfx. CFX96 : $25 / run ETSU $50 / run non ETSU.To schedule training: Contact us at 42 or via email at Use Fee.You must take the introductory course in qPCR offered by the MBCF.Has built-in data analysis modules with automatic baseline subtraction and threshold To Fluorescence Resonance Energy Transfer (FRET) detection. The solid-state optical technology (six filtered LEDs & six filtered photodiodes)Ĭan discriminate up to 5 targets in a single reaction well, and has one channel dedicated Using rapid cycling protocols and fast chemistries to produce results in about an The MBCF is equipped with a Bio-Rad CFX96 Real-time PCR Detection system capable of The reaction, the fluorescent signal increase is directly proportional to the amount Therefore, during this exponential phase of (exponential phase), conditions are optimal and the amount of double-stranded DNA PCR is based on the detection and quantitation of the PCR product as it is made, usingĮither fluorogenic specific probes (such as Taqman probes) or double-stranded DNAīinding fluorescent dyes such as SYBR Green. Unlike conventional PCR that uses an end-point analysis of the amplicon, real-time Services Sample Quantitation and Qualitation Genomics Proteomics Data Acquisition and Imaging Data Analysis Other Services.
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